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MCM6 (Proliferation Marker) Antibody [MCM6/3000]

In Stock
Catalog Number Formulation Size Price
4175-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4175-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4175-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Chromosome, Nucleus
Gene Name
Positive Control
A-431, Human skin or squamous cell carcinoma (IHC).
Immunogen
Recombinant fragment (around aa 228-368) of human MCM6 protein (exact sequence is proprietary)
Alternate Names
DNA replication licensing factor MCM6, p105MCM, DNA replication licensing factor MCM6; MCM6 minichromosome maintenance deficient 6; MIS5 homolog; p105MCM

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
MCM6/3000
Chromosome Location
2q21.3
Mol. Weight of Antigen
105kDa

Functions

  • Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Key References

  • Koonin, E.V. 1993. Nucleic Acids Res. 21: 2541-2547.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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