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KNG1 / Kininogen 1 (KNG1-Heavy chain) Antibody [KNG1/7422]

In Stock
Catalog Number Formulation Size Price
3827-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3827-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3827-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Kininogen is a 644 amino acid precursor protein that is expressed by the KNG1 gene and is secreted into blood plasma. Due to alternative splicing events, several Kininogen protein derivatives exist, including Kininogen LC and Kininogen HC, both of which are produced from the Kininogen precursor and exhibit different functions throughout the cell. Kininogen HC plays an important role in blood coagulation by helping to ensure that prekallikrein and Factor XI (both of which are involved in blood coagulation) are properly situated for interaction with Factor XII. Kininogen HC releases a smaller, active protein known as bradykinin, which plays a role in smooth muscle contraction, induction of hypotension, regulation of blood glucose levels, stimulation of nociceptors and overall mediation of inflammatory responses throughout the cell. In contrast to Kininogen HC, which is involved in blood clotting, Kininogen LC is primarily associated with inhibition of thrombocyte aggregation and also functions as a strong inhibitor of cysteine proteinases.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Extracellular. Secreted. Plasma.
Gene Name
Positive Control
Distinct positivity in plasma and in several cases extracellular matrix along with cytoplasmic expression in kidney.
Immunogen
Recombinant fragment (around aa1-200) of human KNG1 protein (exact sequence is proprietary)
Alternate Names
Kininogen-1 | Kininogen-1 heavy chain | T-kinin | Bradykinin | Lysyl-bradykinin | Kininogen-1 light chain | Low molecular weight growth-promoting factor, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, Ile-Ser-Bradykinin, Kallidin I, Kallidin II, Alpha-2-thiol proteinase inhibitor; BDK; BK; Bradykinin included; Fitzgerald factor; FLAUJEAC FACTOR; HMW kininogen; Ile-Ser-Bradykinin; Kallidin I; Kallidin II; LMW growth-promoting factor; Williams-Fitzgerald-Flaujeac factor

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
KNG1/7422
Chromosome Location
3q27.3
Mol. Weight of Antigen
64 kDa (heavy chain); 53 kDa (light chain)

Functions

  • Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).

Key References

  • Kato, H., et al. 1979. Role of bovine high-molecular-weight (HMW) Kininogen in contact-mediated activation of bovine Factor XII. Adv. Exp. Med. Biol. 120: 19-37.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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