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Kindlin-2 / KIND2 Antibody [3A3]

Additional information

Catalog Number: 10979-MSM1
Reactivity:

, , , ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
10979-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10979-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10979-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Kindlin-2 / KIND2 Antibody

Formalin-fixed, paraffin-embedded human liver stained with KIND2 Mouse Monoclonal Antibody (3A3).

Kindlin-2 / KIND2 Antibody - Image 2

Formalin-fixed, paraffin-embedded human liver stained with KIND2 Mouse Monoclonal Antibody (3A3).

FERMT2 Antibody in SDS-PAGE

FERMT2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Fermitin family homolog 2 Mouse Monoclonal Antibody (3A3). Confirmation of Purity and Integrity of Antibody.

  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Bovine, Hamster, Human, Mouse, Rat
Clone
3A3
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell cortex, Cell junction, Cell projection, Cell surface, Cytoplasm, Cytoskeleton, Focal adhesion, I band, Lamellipodium membrane, Membrane, Myofibril, Nucleus, Sarcomere, Stress fiber
Gene Name
FERMT2
Positive Control
293T whole cell lysate. Human tonsil or liver.
Immunogen
Recombinant protein containing MIG-2 (residues 218-486)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.
Key References
  • Yizeng et al. Cell (2003) 113(1): 37-47
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
10979
SwissProt
Q96AC1
Chromosome Location
14q22.1
Mol. Weight of Antigen
78kDa
Alternate Names
Fermitin family homolog 2, Kindlin-2, Mitogen-inducible gene 2 protein, Pleckstrin homology domain-containing family C member 1, Fermitin family homolog 2 (Fermt2); Kindlin-2 (KIND2); Mitogen-inducible gene 2 protein (MIG2); Pleckstrin homology domain containing family C (with FERM domain) member 1 (PLEKHC1);

Summary

Kindlin-2 (also known as Mig-2, KIND2) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

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