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Golgi Complex (Marker for Human Cells) Antibody [AE-6]

In Stock
Catalog Number Formulation Size Price
MSM2-868-CF700-100T
Purified Ab conjugated to CF700
0.5ml at 100ug/ml
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
golgi-complex-in-cytoplasm, N/A
Gene Name
N/A
Positive Control
HePG2
Immunogen
SU-DHL-1 large cell lymphoma cells
Alternate Names
N/A, N/A, Golgi Complex; Golgi apparatus; Golgi zone

Database Links

Entrez Gene ID
SwissProt
N/A

Additional Information

Clone
AE-6
Chromosome Location
Not Known
Mol. Weight of Antigen
Not Known

Functions

  • N/A

Key References

  • Immunofluorescence Analysis of HeLa cells labeling Fascin with Fascin-1 Mouse Monoclonal Antibody (FSCN1/417)-CF640 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red)

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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