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Glyoxalase 1 (GLO1) Antibody [CPTC-GLO1-3]

In Stock
Catalog Number Formulation Size Price
2739-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2739-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2739-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

GLO1 is an enzyme involved in the detoxification of methylgyoxal, a byproduct of glycolysis. GLO1 expression has been demonstrated by several studies to be upregulated in various human malignant tumors, including metastatic melanoma and lung carcinoma, and thus is a target for pharmaceutical development.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 /
Gene Name
Positive Control
HeLa or Raji cell lysates. Human ovarian carcinoma or prostate carcinoma.
Immunogen
Recombinant full-length human GLO1 protein
Alternate Names
Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, Aldoketomutase; epididymis secretory protein Li 74; Glo1; Glx I; glyoxalase 1 complex; glyoxalase 1 regulatory; glyoxalase 1 structural; glyoxalase domain containing 1; Glyoxalase I; glyoxylase 1; Ketone-aldehyde mutase; lactoyl glutathione lyase; Lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
CPTC-GLO1-3
Chromosome Location
6p21.2
Mol. Weight of Antigen
24-26kDa

Functions

  • Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione (PubMed:20454679, PubMed:9705294, PubMed:23122816). Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B (PubMed:19199007). Required for normal osteoclastogenesis (By similarity).

Key References

  • Sakamoto, H, et al. Clinical Cancer Research U.S. National Library of Medicine, Aug. 2001

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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