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Glyoxalase 1 (GLO1) Antibody [CPTC-GLO1-3]

Additional information

Catalog Number: 2739-MSM2
Gene:

Reactivity:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2739-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2739-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2739-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Glyoxalase 1 (GLO1) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Glyoxalase 1 (GLO1) Mouse Monoclonal Antibody (CPTC-GLO1-3).

Glyoxalase 1 (GLO1) Antibody - Image 2

Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Glyoxalase 1 (GLO1) Mouse Monoclonal Antibody (CPTC-GLO1-3).

Glyoxalase 1 (GLO1) Antibody - Image 3

Western Blot Analysis of human HeLa and HePG2 cell lysates using Glyoxalase 1 (GLO1) Mouse Monoclonal Antibody (CPTC-GLO1-3).

Glyoxalase 1 (GLO1) Antibody - Image 4

SDS-PAGE Analysis of Purified Glyoxalase 1 (GLO1) Mouse Monoclonal Antibody (CPTC-GLO1-3). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CPTC-GLO1-3
Isotype
IgG1
Gene Name
GLO1
Positive Control
HeLa or Raji cell lysates. Human ovarian carcinoma or prostate carcinoma.
Immunogen
Recombinant full-length human GLO1 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione (PubMed:20454679, PubMed:9705294, PubMed:23122816). Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B (PubMed:19199007). Required for normal osteoclastogenesis (By similarity).
Key References
  • Sakamoto, H, et al. Clinical Cancer Research U.S. National Library of Medicine, Aug. 2001
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2739
SwissProt
Q04760
Chromosome Location
6p21.2
Mol. Weight of Antigen
24-26kDa
Alternate Names
Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, Aldoketomutase; epididymis secretory protein Li 74; Glo1; Glx I; glyoxalase 1 complex; glyoxalase 1 regulatory; glyoxalase 1 structural; glyoxalase domain containing 1; Glyoxalase I; glyoxylase 1; Ketone-aldehyde mutase; lactoyl glutathione lyase; Lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

Summary

GLO1 is an enzyme involved in the detoxification of methylgyoxal, a byproduct of glycolysis. GLO1 expression has been demonstrated by several studies to be upregulated in various human malignant tumors, including metastatic melanoma and lung carcinoma, and thus is a target for pharmaceutical development.

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