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GLG1 (Golgi Glycoprotein 1) (Marker for Human Cells) Antibody [GLG1/970]

In Stock
Catalog Number Formulation Size Price
2734-MSM1-CF647-100T
Purified Ab conjugated to CF647
0.5ml at 100ug/ml
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Cytoplasm, Cytoskeleton, Golgi apparatus membrane, Golgi outpost, Microtubule organizing center
Gene Name
Positive Control
HePG2
Immunogen
Golgi fraction from human liver cells
Alternate Names
Golgi apparatus protein 1, CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, Golgi sialoglycoprotein MG-160, CFR-1; Cysteine-rich fibroblast growth factor receptor; E-selectin ligand-1 (ESL-1); Golgi Glycoprotein 1 (GLG1); Golgi apparatus protein 1; Golgi sialoglycoprotein MG-160; Slectin, endothelial cell, ligand

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
GLG1/970
Chromosome Location
16q22.3
Mol. Weight of Antigen
134kDa

Functions

  • Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils).

Key References

  • Nakamura et. al. 1995. J. Cell Biol. 131:1715-26.
  • Nakamura et.al. 1997. Cell. 89(3):445-55.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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