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FOLH1 / PSMA (Prostate Epithelial Marker) Antibody [SPM500]

In Stock
Catalog Number Formulation Size Price
2346-MSM3X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2346-MSM3X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2346-MSM3X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. FOLH1 has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
Positive Control
LNCap or HepG2 cells. Prostate Carcinoma.
Immunogen
Recombinant human FOLH1 protein fragment (around aa 232-433) (exact sequence is proprietary)
Alternate Names
Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, Cell growth-inhibiting gene 27 protein (GIG27); Folate hydrolase 1 (FOLH1); Folylpoly-gamma-glutamate carboxypeptidase (FGCP); Glutamate carboxylase II (GCPII); Glutamate carboxypeptidase 2 (GCP2); Glutamate carboxypeptidase II Membrane glutamate carboxypeptidase N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1 or NAALADase); Prostate-specific membrane antigen (PSM or PSMA); Pteroylpoly-gamma-glutamate carboxypeptidase

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
SPM500
Chromosome Location
11p11.12
Mol. Weight of Antigen
100kDa

Functions

  • Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.
  • Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

Key References

  • Israeli, R.S., et al. 1993. Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen. Cancer Res. 53: 227-230.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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