FITC (Fluorescein Isothiocyanate) [SPM395]

Clear

Summary

This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
389.4Da (Molecular Formula: C21H11NO5S)
Immunogen
Fluorescein isothiocyanate (FITC) conjugated to KLH

Database Links

Entrez Gene ID
Unigene
SwissProt
N/A

Additional Information

Clone
SPM395

Known Applications & Suggested Dilutions

  • Confocal Microscopy
  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • In situ Nucleic Acid Hybridization
  • Western Blot
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.

Key References

  • Balogh P, et. al. Journal of Immunological Methods, 1994, 169(1):35-40.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies