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FITC (Fluorescein Isothiocyanate) Antibody [SPM395]

Additional information

Catalog Number: MSM1X-1277
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
MSM1X-1277-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
MSM1X-1277-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
MSM1X-1277-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
FITC Antibody in SDS-PAGE

FITC Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified FITC Mouse Monoclonal Antibody (F4/1). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
All species
Clone
SPM395
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Fluoresceinated proteins in solution or on tissues.
Immunogen
Fluorescein isothiocyanate (FITC) conjugated to KLH
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • N/A
Key References
  • Balogh P, et. al. Journal of Immunological Methods, 1994, 169(1):35-40.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
Not Applicable
SwissProt
N/A
Mol. Weight of Antigen
389.4Da (Molecular Formula: C21H11NO5S)
Alternate Names
N/A, N/A, Fluorescein isothiocyanate, FITC

Summary

This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

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