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EBI3 / Epstein-Barr virus induced 3 Antibody [EBI3/8905]

In Stock
Catalog Number Formulation Size Price
10148-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10148-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10148-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Epstein-Barr virus-induced gene 3 (Ebi3) is a widely expressed homolog to the interleukin IL-12 p40 subunit protein that forms a heterodimer with either IL-12 p35 or an IL-12 p35 homolog, p28, to create a new cytokine (IL-27). IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive but not memory CD4+ T cells. Interferon-b differentially regulates expression of the IL-12 family members p35, p40, p19 and Ebi3 in activated human dendritic cells. Ebi3 may function to antagonize IL-12 and to inhibit the development of a Th1 immune response. Ebi3 is strongly expressed in Hodgkin and Reed-Sternberg cells, independently of the EBV status of the tumor cells. Research suggests that Ebi3 may be an additional component of the repertoire employed by Hodgkin and Reed-Sternberg cells to inhibit an effective anti-tumor or anti-viral immune response. The human Ebi3 gene maps to chromosome 19p13.3 and encodes a secreted glycoprotein that is expressed in spleen and tonsils, and at high levels in full-term placenta.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Secreted.
Gene Name
Positive Control
Human placenta.
Immunogen
Recombinant fragment (around aa 366-466) of human EBI3 protein
Alternate Names
Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, cytokine receptor; EBI3; EBV induced gene 3 protein; Epstein Barr virus induced 3; Epstein Barr virus induced gene 3; Epstein Barr virus induced gene 3 protein; IL27 subunit beta; IL27B; IL35 subunit; interleukin 27 subunit beta

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
EBI3/8905
Chromosome Location
19p13.3
Mol. Weight of Antigen
34 kDa

Functions

  • Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

Key References

  • Devergne, O., et al. 2001. Am. J. Pathol. 159: 1763-1776.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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