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Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Antibody [E3; same as Ks17.E3]

In Stock
Best Seller
Catalog Number Formulation Size Price
3872-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3872-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3872-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with CK17 Mouse Monoclonal Antibody (E3).

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with CK17 Mouse Monoclonal Antibody (E3).

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CK17 Mouse Monoclonal Antibody (E3).

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CK17 Mouse Monoclonal Antibody (E3).

Cytokeratin 17 Antibody in Western Blot (WB)

Cytokeratin 17 Antibody in Western Blot (WB)

Western Blot Analysis of HeLa lysate using Cytokeratin 17 Mouse Monoclonal Antibody (E3; same as Ks17.E3)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glandssuch as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as ‘epithelial stem cell’ marker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Goat, Human, Pig, Rat
Research Areas
Autophagy, Developmental Biology, Basal Cell Marker
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT17
Positive Control
T24 cells. Skin. Bladder or Cervix.
Immunogen
Cytoskeletal fraction of rat colon epithelium
Alternate Names
Keratin, type I cytoskeletal 17, 39.1, Cytokeratin-17, Keratin-17, K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1

Database Links

Entrez Gene ID
3872
SwissProt
Q04695

Additional Information

Clone
E3; same as Ks17.E3
Chromosome Location
17q21.2
Mol. Weight of Antigen
46kDa

Related Antibodies

View All KRT17 Antibodies

Functions

  • Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.

Key References

  • Smedts et. al. Am J Pathol 141: 497, 1992.
  • Smedts et. al. Am J Pathol 140: 601, 1992.
  • Wetzels et. al. Histopathol 20: 295, 1992.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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