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CSPG4 / MCSP (Marker of Malignant Melanoma) Antibody [LHM 2]

Additional information

Catalog Number: 1464-MSM17
Reactivity:

, ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1464-MSM17-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
1464-MSM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1464-MSM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CSPG4 / NG2 Antibody in Immunofluorescence (IF)

CSPG4 / NG2 Antibody in Immunofluorescence (IF)

Confocal immunofluorescence image of HepG2 cells stained with CSPG4 / MCSP Mouse Monoclonal Antibody (LHM 2) followed by goat anti-Mouse CF488 (green). RedDot is used to label the nuclei red.

CSPG4 / MCSP Antibody in SDS-PAGE

CSPG4 / MCSP Antibody in SDS-PAGE

SDS-PAGE Analysis Purified CSPG4 / NG2 Mouse Monoclonal Antibody (LHM 2). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse, Rat
Clone
LHM 2
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Cell projection, Cell surface
Gene Name
CSPG4
Positive Control
MCF7 or HepG2 cells. Human brain or malignant melanoma.
Immunogen
A 375P cells crude extract
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. Valuable marker for several incompletely differentiated precursor cells.
Key References
  • Kupsch, J.M., et al. 1995. Melanoma Res. 5: 403-411.
  • Chen, Xinyi et al. “Chondroitin Sulfate Proteoglycan 4 (CSPG4) as an Emerging Target for Immunotherapy to Treat Melanoma.” Cancers vol. 16,19 3260. 25 Sep. 2024, doi:10.3390/cancers16193260
  • Greiner, Daniel et al. “CSPG4-targeting CAR-macrophages inhibit melanoma growth.” bioRxiv : the preprint server for biology 2024.06.04.597413. 6 Jun. 2024, doi:10.1101/2024.06.04.597413. Preprint.
  • Greiner, Daniel et al. “Human CSPG4-targeting CAR-macrophages inhibit melanoma growth.” Oncogene vol. 44,22 (2025): 1665-1677. doi:10.1038/s41388-025-03332-0
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1464
SwissProt
Q6UVK1
Chromosome Location
15q24.2
Mol. Weight of Antigen
270-300kDa
Alternate Names
Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan, NG2 Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan, Melanoma-associated Chondroitin Sulfate Proteoglycan (MCSP); NG2; CSPG4

Summary

NG2 (also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16 and MEL-CSPG) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with a4 b1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Micro-vessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering.

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