Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Coagulation Factor VII / F7 Antibody [F7/3516]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
2155-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2155-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2155-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor VII (serum prothrombin conversion accelerator, proconvertin, F7, Factor VII) is a 406 amino acid, vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor IX A, Factor X A, Factor XII A or Thrombin-mediated proteolytic cleavage of Factor VII at Arg 152-Ile 153 generates Factor VII A, an active serine protease composed of a catalytic heavy chain disulfide linked to a light chain, containing two EGF-like domains. Mutations at the F7 locus that lead to Factor VII deficiencies are generally asymptomatic or phenotypically uncharacterized, with hemorrhagic diathesis occurring at extremely low levels.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Secreted
Gene Name
F7
Positive Control
Human liver or gastric carcinoma.
Immunogen
Recombinant fragment (around aa 366-466) of human F7 protein
Alternate Names
Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, Eptacog alfa; Factor VII heavy chain; Factor VII light chain; FVII coagulation protein; Proconvertin; Serum prothrombin conversion accelerator (SPCA)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
F7/3516
Chromosome Location
13q34
Mol. Weight of Antigen
200/80/73/50/43 kDa

Functions

  • Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Key References

  • Davie, E.W., et al. 1975. Basic mechanisms in blood coagulation. Annu. Rev. Biochem. 44: 799-829.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Coagulation Factor VII / F7 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK