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Recombinant CDK4 Antibody [CDK4/8210R]

Additional information

Catalog Number: 1019-RBM2
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1019-RBM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1019-RBM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1019-RBM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CDK4 Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CDK4 Recombinant Rabbit Monoclonal Antibody (CDK4/8210R). Inset: PBS instead of primary antibody; secondary only negative control.

CDK4 Antibody in Western Blot (WB)

CDK4 Antibody in Western Blot (WB)

Western blot analysis of MCF7 and HEK293T cell lysates using CDK4 Recombinant Rabbit Monoclonal Antibody (CDK4/8210R).

CDK4 Antibody in Western Blot (WB)

CDK4 Antibody in Western Blot (WB)

Western blot analysis of K-562 cell lysate using CDK4 Recombinant Rabbit Monoclonal Antibody (CDK4/8210R).

CDK4 Antibody in SDS-PAGE

CDK4 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Cyclin-dependent kinase 4 Recombinant Rabbit Monoclonal Antibody (CDK4/8210R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CDK4/8210R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus. Cytoplasm.
Gene Name
CDK4
Positive Control
MCF7, HEK293T, cervical carcinoma and liposarcomas.|Human urothelial carcinoma, K-562
Immunogen
Recombinant fragment corresponding to N-terminal of human CDK4 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Host:

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Host:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Key References
  • Ma L et al. Oncotarget 8:4125-4135 (2017).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1019
SwissProt
P11802
Chromosome Location
12q14.1
Mol. Weight of Antigen
34kDa
Alternate Names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4 protein; CDK4_HUMAN; Cell division protein kinase 4; CMM3; Crk3; Cyclin dependent kinase 4; Melanoma cutaneous malignant 3; MGC14458; p34 cdk4; PSK-J3

Summary

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level (PMID:17253961). CCND1- CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth (PMID:21844184). CDK4 is highly expressed in highly differentiated and dedifferentiated liposarcomas, but rarely expressed in other benign liposarcomas and other sarcomas. CDK4 and MDM2 combined to differentiate between highly differentiated liposarcoma (+), dedifferentiated liposarcoma (+) and myxoid liposarcoma, pleomorphic liposarcoma, spindle lipoma, pleomorphic lipoma and other high-grade sarcomas.

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