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CD54 / ICAM-1 Antibody [15.2]

Additional information

Catalog Number: 3383-MSM3
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3383-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3383-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3383-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD54 / ICAM-1 Antibody

SDS-PAGE Analyis Purified CD54 Mouse Monoclonal Antibody (15.2). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • Functional Studies
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Pig
Clone
15.2
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Membrane
Gene Name
ICAM1
Positive Control
HeLa or SW480 cells. Human tonsil., MOLT 4, Raji cells
Immunogen
Human monocytes.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
  • (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.
  • (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.
  • (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Key References
  • Johnson, J.P., et al. Leucocyte Typing IV, Oxford Univ. Press, pp 681-683. (FC)
  • Hromatka, BS et al. Biol Reprod. 2013 Jun 20;88(6):154. (IHC)
  • Huegel R et al. J Invest Dermatol. 2007 Jan;127(1):65-74. (IC)
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3383
SwissProt
P05362
Chromosome Location
19p13.3-p13.2
Mol. Weight of Antigen
85-115kDa
Alternate Names
Intercellular adhesion molecule 1, Major group rhinovirus receptor, CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

Summary

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. ICAM-1 may also be related to progression and metastasis of tumors.

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