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CD3e (T-Cell Marker) Antibody [B-B12]

In Stock
Catalog Number Formulation Size Price
916-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
916-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
916-MSM3-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow Cytometric Analysis of Jurkat cells. CD3e Mouse Monoclonal Antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of Jurkat cells. CD3e Mouse Monoclonal Antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (B-B12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

SDS-PAGE Analysis of Purified CD3e Mouse Monoclonal Antibody (B-B12). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD3e Mouse Monoclonal Antibody (B-B12). Confirmation of Integrity and Purity of Antibody.

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 mouse monoclonal antibody (B-B12) (green).

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 mouse monoclonal antibody (B-B12) (green).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Cardiovascular, Immuno Oncology, Immunology, Hematopoietic Stem Cells, PD-1 blockade immunotherapy
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
CD3E
Positive Control
Jurkat cells. Tonsil and lymph node.
Immunogen
Human T cell leukemia cells
Alternate Names
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Database Links

Entrez Gene ID
916
SwissProt
P07766

Additional Information

Clone
B-B12
Chromosome Location
11q23.3
Mol. Weight of Antigen
20kDa

Related Antibodies

View All CD3E Antibodies

Functions

  • Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

Key References

  • Clevers et al., Ann. Rev. Immunol. 6, 629, (1988)
  • Oettgen and Terhorst, Hum. Immunol. 18, 187, (1987)
  • Kishimoto T. et al., eds. Leukocyte Typing VI, p44-48 and p1111, Garland Publishing, Inc, New York and London, 1997.Furley et al., Cell 46, 75, (1986)
  • Gold et al., Nature 321, 4, (1986)

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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