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CD151 Antibody [11G5a]

In Stock
Catalog Number Formulation Size Price
977-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
977-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
977-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

CD151 is involved in a wide variety of cell biological processes, including cell adhesion and the transport of integrins via vesicles. The human CD151 gene maps to chromosome 11p15.5 and encodes a 253 amino acid protein, which belongs to the tetraspan (4TM) superfamily. CD151 can associate with several integrin chains including 1, 3, 4, 伪2, 伪3, 伪5 and 伪6 integrins. CD151 may provide a framework for the spatial organization of both type I and type II hemidesmosomes, which are specialized junctional complexes that function as cell attachment sites for binding to basement membranes. CD151 RNA transcript (1.6 kb) can be detected in MO7e cells, bone marrow stromal cells, C11 endothelial cells, HUVEC and several myeloid leukemia cell lines, however, no transcript is detected in brain and the lymphoblastoid cell lines MOLT-4 and BALM-1. Leu 149-Glu 213 of CD151 is the interface through which Integrin 伪3/ 1 can bind. CD151 can enhance cell motility, invasion and metastasis of cancer cells in a focal adhesion kinase dependent manner.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
Positive Control
Human tonsil or spleen. HT1080 cells.
Immunogen
The details of the immunogen for this antibody are not available.
Alternate Names
CD151 antigen, GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3, Tetraspanin-24, GP27; hemidesmosomal tetraspanin CD151; Membrane glycoprotein SFA-1; MER2; Platelet endothelial tetraspan antigen 3 (PETA3); platelet surface glycoprotein gp27; Platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; Red blood cell antigen MER2; SFA1; Tetraspanin-24 (TSPAN24)

Database Links

Entrez Gene ID
977
SwissProt

Additional Information

Clone
11G5a
Chromosome Location
11p15.5
Mol. Weight of Antigen
28-32kDa

Functions

  • Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
  • (Microbial infection) Plays a role in human papillomavirus 16/HPV-16 endocytosis upon binding to cell surface receptor.

Key References

  • Zhu, G.H., et al. 2011. Expression and prognostic significance of CD151, c-Met, and integrin α3/α6 in pancreatic ductal adenocarcinoma. Dig. Dis. Sci. 56: 1090-1098.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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