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CD147 / BSG / EMMPRIN / Neurothelin Antibody [BSG/7955]

Additional information

Catalog Number: 682-MSM15
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
682-MSM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
682-MSM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
682-MSM15-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD147 Antibody in Immunohistochemistry (IHC (P))

CD147 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human placenta stained with CD147 Mouse Monoclonal Antibody (BSG/7955) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

CD147 Antibody in Immunohistochemistry (IHC (P))

CD147 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with CD147 Mouse Monoclonal Antibody (BSG/7955) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

CD147 Antibody in Western Blot (WB)

CD147 Antibody in Western Blot (WB)

Western Blot Analysis of HeLa cell lysate using CD147 Mouse Monoclonal Antibody (BSG/7955).

CD147 Antibody in SDS-PAGE

CD147 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD147 Mouse Monoclonal Antibody (BSG/7955). Confirmation of Integrity and Purity of Antibody.

CD147 Antibody in Western Blot (WB)

CD147 Antibody in Western Blot (WB)

Western Blot Analysis of HepG2, HeLa and MCF7 cell lysates using CD147 Mouse Monoclonal Antibody (BSG/7955).

CD147 Antibody in Western Blot (WB)

CD147 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Human Heart, Human Intestine, and Human Kidney tissue lysates using CD147 Mouse Monoclonal Antibody (BSG/7955).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
BSG/7955
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell surface
Gene Name
BSG
Positive Control
HeLa cells. Human renal cell carcinoma, ovarian carcinoma or melanoma. HepG2, MCF7, Human Brain, Human Heart, Human Intestine, Human Kidney
Immunogen
Recombinant full-length human BSG protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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Formalin-fixed, paraffin-embedded Human Renal Cell Carcinoma Stained with KSP-Cadherin Rabbit Polyclonal Antibody.
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  • IHC

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Additional information

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Reactivity:

, ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (SPM594).
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Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

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Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2618).
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Additional information

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Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2616).
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Additional information

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Gene:

Host:

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Additional information

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Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).
  • (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2.
  • Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed:11943775, PubMed:11688976). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed:17127621, PubMed:21778275, PubMed:28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed:25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed:19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed:12553375, PubMed:11992541, PubMed:15833850).
  • (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3 (PubMed:22080952, PubMed:26195724). Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed:28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed:28409866).
  • (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA.
  • (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA.
  • (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection.
  • (Microbial infection) Acts as a receptor for measles virus.
  • (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells.
Key References
  • Kirsch AH et. al. Tissue Antigens. 1997 Aug;50(2):147-52.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
682
SwissProt
P35613
Chromosome Location
19p13.3
Mol. Weight of Antigen
35kDa (non-reduced); 40kDa (reduced)
Alternate Names
Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, 5A11 Antigen; 5F7; Basigin; Blood brain barrier HT7 antigen BSG; Extracellular Matrix Metalloproteinase Inducer (EMMPRIN); Leukocyte Activation Antigen M6; Neurothelin; OK Blood Group Antigen; TCSF; Tumor Cell-derived Collagenase Stimulatory Factor

Summary

This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

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