Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Printable Datasheet

Recombinant CD14 (Monocyte / Macrophage Marker) Antibody [LPSR/4180R]

In Stock
Catalog Number Formulation Size Price
929-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
929-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
929-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD14 is receptor for endotoxin and apoptotic cells. It is expressed by monocytes/histiocytes, Langerhans' cells, follicular dendritic reticulum cells, neutrophils (weakly), and endothelial cells of the liver sinusoids.CD14 is detected in myeloid leukemias with monoblastic/monocytic differentiation, and in histiocytic neoplasms like histiocytic lymphoma, Langerhans' cell histiocytosis and giant cell tumor. In cultures, a subpopulation of Kaposi sarcoma cells express CD14. In differential diagnosis, CD14 is more specific than CD68 in the identification of monocytes/histiocytes and associated neoplasias.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, B Cell Markers, Dendritic Cell Marker, Hematopoietic Stem Cells, Infectious Disease, MAPK Signaling, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Golgi apparatus, Membrane raft, Secreted
Gene Name
CD14
Positive Control
Human lymph node or tonsil.
Immunogen
Synthetic peptide corresponding to residues withinaa1-100 of CD14 was used as an immunogen.
Alternate Names
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, Lipopolysaccharide receptor; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein

Database Links

Entrez Gene ID
929
SwissProt
P08571

Additional Information

Clone
LPSR/4180R
Chromosome Location
5q31.1
Mol. Weight of Antigen
40kDa

Related Antibodies

View All CD14 Antibodies

Functions

  • Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).

Key References

  • Simmons, D.L., et al. 1989. Blood 73: 284-289.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant CD14 (Monocyte / Macrophage Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales