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Recombinant CD14 (Monocyte / Macrophage Marker) Antibody [LPSR/4180R]

In Stock
Catalog Number Formulation Size Price
929-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
929-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
929-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD14 is receptor for endotoxin and apoptotic cells. It is expressed by monocytes/histiocytes, Langerhans' cells, follicular dendritic reticulum cells, neutrophils (weakly), and endothelial cells of the liver sinusoids.CD14 is detected in myeloid leukemias with monoblastic/monocytic differentiation, and in histiocytic neoplasms like histiocytic lymphoma, Langerhans' cell histiocytosis and giant cell tumor. In cultures, a subpopulation of Kaposi sarcoma cells express CD14. In differential diagnosis, CD14 is more specific than CD68 in the identification of monocytes/histiocytes and associated neoplasias.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, B Cell Markers, Dendritic Cell Marker, Hematopoietic Stem Cells, Infectious Disease, MAPK Signaling, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Golgi apparatus, Membrane raft, Secreted
Gene Name
CD14
Positive Control
Human lymph node or tonsil.
Immunogen
Synthetic peptide corresponding to residues withinaa1-100 of CD14 was used as an immunogen.
Alternate Names
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, Lipopolysaccharide receptor; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein

Database Links

Entrez Gene ID
929
SwissProt
P08571

Additional Information

Clone
LPSR/4180R
Chromosome Location
5q31.1
Mol. Weight of Antigen
40kDa

Related Antibodies

View All CD14 Antibodies

Functions

  • Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).

Key References

  • Simmons, D.L., et al. 1989. Blood 73: 284-289.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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