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Calbindin 1 (CALB1) Antibody [MSVA-471M]

Additional information

Catalog Number: 793-MSM21
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
793-MSM21-P0
Purified Ab with BSA and Azide
20ug
$259.00
793-MSM21-P1
Purified Ab with BSA and Azide
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CALB1 Antibody in Numerous Tissues (IHC)

CALB1 Antibody in Numerous Tissues (IHC)

Calbindin Mouse Monoclonal Antibody (MSVA-471M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:50-1:100 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-471M
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
CALB1
Positive Control
Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining.
Immunogen
Recombinant fragment (around aa 7-96) of human CALB1 protein (exact sequence is proprietary)
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab purified from Bioreactor Concentrate by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Other Information

Functions
  • Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Key References
  • Parmentier, M., et al. 1989. Genomics 4: 309-319.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
793
SwissProt
P05937
Chromosome Location
8q21.3
Mol. Weight of Antigen
52kDa
Alternate Names
Calbindin, Calbindin D28, D-28K, Vitamin D-dependent calcium-binding protein, avian-type, avian-type; CAB27; CALB 1; CALB; CALB1; CALB1_HUMAN; Calbindin 1 28kDa; Calbindin; Calbindin D28; D 28K; D-28K; D28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL H protein; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D-dependent calcium-binding protein

Summary

The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 (also designated oncomodulin). Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261-amino acid protein with 6 EF-hand domains, 4 of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function.

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