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C1QA / Complement C1q A-Chain Antibody [C1QA/2953]

In Stock
Catalog Number Formulation Size Price
712-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
712-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
712-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

C1q, a subcomponent of the classical complement pathway, is composed of nine subunits that mediate classical complement activation and thereby play an important role in the immune response. Six of these subunits are disulfide-linked dimers of chains A and B, while three of these subunits, designated C1q-A through C1q-C, are disulfide-linked dimers of chain C. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. The presence of receptors for C1q on effector cells modulates its activity, which may be antibody-dependent or independent. Macrophages are the primary source of C1q, while anti-inflammatory drugs as well as cytokines differentially regulate expression of the mRNA as well as the protein. C1q deficiency is associated with lupus erythematosus and glomerulonephritis.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Secreted
Gene Name
Positive Control
Human liver or kidney tissue (IHC). T-47D, PC-3 cell lysate (WB).
Immunogen
Recombinant fragment (around aa 104-237) of human C1QA protein (exact sequence is proprietary)
Alternate Names
Complement C1q subcomponent subunit A, Complement C1q subcomponent subunit A; Complement component 1 q subcomponent alpha polypeptide

Database Links

Entrez Gene ID
712
SwissProt

Additional Information

Clone
C1QA/2953
Chromosome Location
1p36.12
Mol. Weight of Antigen
29kDa

Functions

  • C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Key References

  • Kishore, U., et al. 2000. C1q: structure, function and receptors. Immunopharmacology 49: 159-170.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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