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Bax (Apoptosis Marker) Antibody [SPM336]

Additional information

Catalog Number: 581-MSM1X
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
581-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
581-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
581-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Bax (Apoptosis Marker) Antibody

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Bax Monoclonal Antibody (Clone SPM33).

Bax (Apoptosis Marker) Antibody - Image 2

Flow Cytometric Analysis of human Jurkat cells using BAX Mouse Monoclonal Antibody (2D2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Bax (Apoptosis Marker) Antibody - Image 3

Immunofluorescence Analysis of Jurkat cells labeling BAX with Bax Monoclonal Antibody (Clone SPM33) followed by Goat anti-Mouse IgG-CF488 (Green).The nuclear counterstain is NucSpot®.

BAX Antibody in SDS-PAGE

BAX Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Apoptosis regulator BAX Mouse Monoclonal Antibody (SPM336). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Monkey
Clone
SPM336
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm, Mitochondrion outer membrane
Gene Name
BAX
Positive Control
HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin s lymphoma., Jurkat, K562
Immunogen
A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:16113678, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816, PubMed:11060313, PubMed:16199525). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:11060313, PubMed:16199525, PubMed:10772918, PubMed:16113678, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:11060313, PubMed:16199525, PubMed:10772918, PubMed:16113678, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816).
Key References
  • Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34.
  • Hsu YT, et. al. PNAS, 1997, 94(8):3668-72.
  • Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
581
SwissProt
Q07812
Chromosome Location
19q13.33
Mol. Weight of Antigen
21kDa
Alternate Names
Apoptosis regulator BAX, Bcl-2-like protein 4, Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

Summary

Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

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