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ARF1 (Golgi Apparatus Marker) Antibody [3F1]

In Stock
Catalog Number Formulation Size Price
375-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
375-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
375-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified ARF1 Mouse Monoclonal Antibody (3F1). Confirmation of Integrity and Purity of the Antibody.

SDS-PAGE Analysis of Purified ARF1 Mouse Monoclonal Antibody (3F1). Confirmation of Integrity and Purity of the Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse, Rat
Research Areas
Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell junction, Cytoplasm, Golgi apparatus, Membrane, Perinuclear region, Postsynaptic density, Synapse, Synaptosome, trans-Golgi network membrane
Gene Name
ARF1
Positive Control
Brain or Breast., HeLa cells. Kidney, Liver
Immunogen
Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.
Alternate Names
ADP-ribosylation factor 1, ADP-ribosylation factor 1; ARF1

Database Links

Entrez Gene ID
375
SwissProt
P84077

Additional Information

Clone
3F1
Chromosome Location
1q42.13
Mol. Weight of Antigen
20kDa

Related Antibodies

View All ARF1 Antibodies

Functions

  • GTP-binding protein involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
  • (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

Key References

  • Austin et al. 2000. J Biol Chem. 275(29):21862-9.
  • Puxeddu et al. 2009. Proc Natl Acad Sci U S A. 106(15):6158-63.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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