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ALDH1L1 Antibody [ALDH1L1/7702]

Additional information

Catalog Number: 10840-MSM2
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
10840-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10840-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10840-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
ALDH1L1 Antibody in SDS-PAGE

ALDH1L1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified ALDH1L1 Mouse Monoclonal Antibody (ALDH1L1/7702). Confirmation of Purity and Integrity of Antibody.

ALDH1L1 Antibody in Western Blot (WB)

ALDH1L1 Antibody in Western Blot (WB)

Western blot analysis of A549 cell lysate using ALDH1L1 Mouse Monoclonal Antibody (ALDH1L1/7702).

ALDH1L1 Antibody in Immunohistochemistry (IHC (P))

ALDH1L1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with ALDH1L1 Mouse Monoclonal Antibody (ALDH1L1/7702). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

ALDH1L1 Antibody in Immunohistochemistry (IHC (P))

ALDH1L1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human colon stained with ALDH1L1 Mouse Monoclonal Antibody (ALDH1L1/7702). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
ALDH1L1/7702
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
ALDH1L1
Positive Control
Human kidney or liver. A549 or SK-BR3 cells.
Immunogen
Recombinant fragment (around aa400-600) of human ALDH1L1 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).
Key References
  • Champion, K.M., et al. 1994. Identification of a heritable deficiency of the folate-dependent enzyme 10-formyltetrahydrofolate dehydrogenase in mice. Proc. Natl. Acad. Sci. USA 91: 11338-11342.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
10840
SwissProt
O75891
Chromosome Location
3q21.3
Mol. Weight of Antigen
99kDa
Alternate Names
Cytosolic 10-formyltetrahydrofolate dehydrogenase, Aldehyde dehydrogenase family 1 member L1, 10-FTHFDH; Aldehyde dehydrogenase family 1 member L1; aldh1l1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; EC 1.5.1.6; FDH; formyltetrahydrofolate dehydrogenase; FTHFD

Summary

Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde, lipid peroxidation and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH1L1 (aldehyde dehydrogenase 1 family member L1), also known as FTHFD or 10-FTHFD (10-formyltetrahydrofolate dehydrogenase), is a cytosolic protein that is developmentally regulated in the cerebellum. ALDH1L1 binds to folate and catalyzes the conversion of 10-formyltetrahydrofolate (10-FTHF) to tetrahydrofolate (THF). This suggests a possible role for ALDH1L1 in the regulation of cellular THF levels as well as in the inhibition of cell proliferation (as 10-FTHF is essential for the synthesis of purine). In addition, the overexpression of ALDH1L1 can restrict cell proliferation in vitro.

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