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Home >> Antibodies >> L-Myc / MYCL1 (Transcription Factor)

L-Myc / MYCL1 (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-MYCL-2D5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified L-Myc / MYCL Mouse Monoclonal Antibody (PCRP-MYCL-2D5). Confirmation of Integrity and Purity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. L-Myc / MYCL Mouse Monoclonal Antibody (PCRP-MYCL-2D5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using L-Myc / MYCL Mouse Monoclonal Antibody (PCRP-MYCL-2D5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using L-Myc / MYCL-Monospecific Mouse Monoclonal Antibody (PCRP-MYCL-2D5).

Product Details

Synonyms

bHLHe38; Class E basic helix-loop-helix protein 38; l myc 1 proto-oncogene; Myc related gene from lung cancer; oncogene lmyc; Protein L-Myc-1; v myc avian myelocytomatosis viral oncogene homolog 1 lung carcinoma derived

Positive Control

HeLa cells. Human small cell lung carcinoma.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MYCL protein

Cellular Localization

Nucleus. Nucleoplasm. Mitotic chromosome.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

40.33kDa

Specificity & Comments

Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas, and the L-Myc gene in human small cell lung carcinomas. c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 4610
  •  Gene Symbol: MYCL
  •  SwissProt: P12524
  •  Chromosome Location: 1p34.2
  •  Unigene: 437922