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Myeloperoxidase / MPO Antibody [MSVA-692M]

Additional information

Catalog Number: 4353-MSM14
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
4353-MSM14-P0
Purified Ab with BSA and Azide
20ug
$324.50
4353-MSM14-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MPO Antibody in Numerous Tissues (IHC)

MPO Antibody in Numerous Tissues (IHC)

Myeloperoxidase Mouse Monoclonal Antibody (MSVA-692M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-692M
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Lysosome
Gene Name
MPO
Positive Control
Spleen: Numerous granulocytes with strong MPO staining should be seen within the red pulp of the spleen.
Immunogen
Recombinant fragment of human MPO protein (exact sequence is proprietary)
Expression System
CHO
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Key References
  • Toth B, Wehrmann M,et al. J Clin Pathol 1999 Sep;52(9):688-92.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4353
SwissProt
P05164
Chromosome Location
17q22
Mol. Weight of Antigen
heavy-light promoter: 72kDa; dimer: 140kDa
Alternate Names
Myeloperoxidase, 84 kDa / 89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; MPO; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain

Summary

Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles). With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.

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