Recombinant Calponin-1 (Smooth Muscle Marker) Antibody [MSVA-455R]

Additional information

Catalog Number: 1264-RBM16
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
1264-RBM16-P0
Purified Ab with BSA and Azide
20ug
$324.50
1264-RBM16-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • IHC

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Host
Rabbit
Applications
Species Reactivity
Clone
MSVA-455R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
Positive Control
Colon: A moderate to strong Calponin 1 staining should be seen in the muscularis mucosae, muscularis propria and in the walls of larger blood vessels.
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human Calponin was used as an immunogen
Expression System
HEK293
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
SwissProt
Chromosome Location
19p13.2
Mol. Weight of Antigen
33kDa
Alternate Names
Calponin-1, Basic calponin, Calponin H1, smooth muscle, Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin-1; CNN1; Cnn1; Sm Calp; SMCC

Summary

Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.

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