| Catalog Number: | 2670-MSM7 |
|---|---|
| Gene: | |
| Host: | |
| Reactivity: | Guinea Pig, Hamster, Human, Mouse, Rat |
Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Recombinant Monoclonal Antibody (rASTRO/789).
Western Blot Analysis of human brain tissue lysate using GFAP Mouse Recombinant Monoclonal Antibody (rASTRO/789).
Flow Cytometric Analysis of T98G cells. GFAP Recombinant Mouse Monoclonal Antibody (rASTRO/789) followed by goat anti-mouse IgG-CF488 (blue); Isotype Control (red).
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea Pig Brain and Hamster Brain tissue lysates using GFAP Mouse Recombinant Monoclonal Antibody (rASTRO/789).
SDS-PAGE Analysis of Purified Glial fibrillary acidic protein Recombinant Mouse Monoclonal Antibody (rASTRO/789). Confirmation of Purity and Integrity of Antibody.
Mouse Monoclonal Antibody GFAP antibody (GFAP/15097) was utilized as the capture antibody, coated onto the microtiter plate at 1 µg/mL. A biotinylated Anti-GFAP clone (rASTRO/789) was used as the detection antibody at a 1:2000 dilution. The graph displays background-subtracted absorbance values measured at 450 nm across a serial dilution of the target antigen.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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