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YY1 (Transcription Factor) Antibody [YY1/5436]

In Stock
Catalog Number Formulation Size Price
7528-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7528-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7528-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsil stained with YY1 Mouse Monoclonal (YY1/5436). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with YY1 Mouse Monoclonal (YY1/5436). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified YY1 Mouse Monoclonal Antibody (YY1/5436). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified YY1 Mouse Monoclonal Antibody (YY1/5436). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Daudi cell lysate using YY1 Mouse Monoclonal (YY1/5436).

Western blot analysis of Daudi cell lysate using YY1 Mouse Monoclonal (YY1/5436).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal end. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3' enhancer and immunoglobulin heavy-chain �E1 site as well as the P5 promoter of the adenoassociated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, Developmental Biology, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Nucleus matrix. Associated with the nuclear matrix.
Gene Name
YY1
Positive Control
Human kidney|tonsil or cervix carcinoma tissues
Immunogen
His-tagged YY1 protein
Alternate Names
Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, CF1; Delta transcription factor; INO80 complex subunit S; NF E1; NFE1; Transcriptional repressor protein YY1; UCR motif DNA binding protein; UCRBP; Yin and yang 1; Yin and Yang 1 protein; YY-1; YY1 transcription factor

Database Links

Entrez Gene ID
7528
SwissProt
P25490

Additional Information

Clone
YY1/5436
Chromosome Location
14q32.2
Mol. Weight of Antigen
40/68kDa

Related Antibodies

View All YY1 Antibodies

Functions

  • Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence (PubMed:1655281). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed (PubMed:11158321). Involved in DNA repair (PubMed:18026119, PubMed:28575647). In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation (PubMed:28575647).
  • Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.

Key References

  • Shi, Y., et al. 1991. Transcriptional repression by YY1, a human GLI-Kruppelrelated protein, and relief of repression by adenovirus E1A protein. Cell 67: 377-388.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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