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Sulfotransferase family 1E, estrogen-preferring, member 1 Antibody [SULT1E1/7124]

Additional information

Catalog Number: 6783-MSM4
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6783-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6783-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6783-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Sulfotransferase family 1E, estrogen-preferring, member 1 Antibody

Formalin-fixed, paraffin-embedded human small intestine stained with SULT1E1 Mouse Monoclonal Antibody (SULT1E1/7124). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Sulfotransferase family 1E, estrogen-preferring, member 1 Antibody - Image 2

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with SULT1E1 Mouse Monoclonal Antibody (SULT1E1/7124). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SULT1E1 Antibody in SDS-PAGE

SULT1E1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Sulfotransferase 1E1 Mouse Monoclonal Antibody (SULT1E1/7124). Confirmation of Purity and Integrity of Antibody.

SULT1E1 Antibody in Western Blot (WB)

SULT1E1 Antibody in Western Blot (WB)

Western Blot Analysis of Intestine, Liver and Skin lysates of human tissue using SULT1E1 Mouse Monoclonal Antibody (SULT1E1/7124).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
SULT1E1/7124
Isotype
IgG2
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
SULT1E1
Positive Control
HEPG2 cells. Human liver|intestine or kidney. Skin.
Immunogen
Recombinant fragment (around aa100-294) of human SULT1E1 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone (PubMed:7779757, PubMed:11884392, PubMed:11006110). Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency (PubMed:11006110, PubMed:19589875). Does not sulfonate cortisol, testosterone and dopamine (PubMed:7779757, PubMed:11006110). May play a role in gut microbiota-host metabolic interaction. O-sulfonates 4-ethylphenol (4-EP), a dietary tyrosine-derived metabolite produced by gut bacteria. The product 4-EPS crosses the blood-brain barrier and may negatively regulate oligodendrocyte maturation and myelination, affecting the functional connectivity of different brain regions associated with the limbic system.
Key References
  • Rebbeck, T.R., et al. 2006. J. Natl. Cancer Inst. 98: 1311-1320.
  • Allali-Hassani, A., et al. 2007. PLoS Biol. 5: e97.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6783
SwissProt
P49888
Chromosome Location
14q13.3
Mol. Weight of Antigen
35kDa
Alternate Names
Sulfotransferase 1E1, EST-1, Estrogen sulfotransferase, Sulfotransferase, estrogen-preferring, EST; EST-1;EST1; Estrogen sulfotransferase; estrogen-preferring; estrone sulfotransferase; ST1E1; STE; Sulfotransferase 1E1; Sulfotransferase ; Sulfotransferase estrogen preferring

Summary

The soluble sulfotransferases contribute to the elimination of xenobiotics, the activation of procarcinogens and the regulation of hormones by catalyzing the sulfate conjugation of these substances. SULT1E1, also known as STE, is a 294 amino acid member of the SULT1 family. Localized to the cytoplasm and expressed in intestine, liver and kidney, SULT1E1 exists as a homodimer that is thought to control estrogen receptor (ER) levels by sulfurylating free estradiol. Defects in the gene encoding SULT1E1 are associated with an increased risk for endometrial cancer, suggesting a role for SULT1E1 in tumorigenesis.

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