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Recombinant SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody [SDHB/8974R]

Additional information

Catalog Number: 6390-RBM9
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6390-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6390-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6390-RBM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/8974R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDHB Antibody in Western Blot (WB)

SDHB Antibody in Western Blot (WB)

Western blot analysis of HepG2 lysate using SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/8974R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
SDHB/8974R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm. Mitochondria.
Gene Name
SDHB
Positive Control
Jurkat or HepG2 cells. Human kidney or heart tissue.
Immunogen
Synthetic peptide corresponding to residues in human SDHB was used as an immunogen.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Key References
  • Chang HC et al. Redox Biol 20:321-333 (2019).
  • Her YF et al. PLoS One 10:e0127471 (2015).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6390
SwissProt
P21912
Chromosome Location
1p36.13
Mol. Weight of Antigen
32kDa
Alternate Names
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Iron-sulfur subunit of complex II, CWS2; DHSB; Iron-sulfur subunit of complex II; PGL4; SDH2; SDHB; SDHIP; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein

Summary

Succinate dehydrogenase B (SDHB) is an iron-sulfur subunit of mitochondrial complex II, a key component of the citric acid cycle and the electron transport chain, a respiratory complex that catalyzes the oxidation of succinate in the mitochondrial membrane.Many cancers are generally positive for SDHB, including renal cell carcinomas and gastrointestinal stromal tumors. A subset of RCC and GIST tumors that are associated with SDH mutations, Carney-Stratakis Syndrome or Carney Triad exhibit a loss of SDHB expression.

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