Replication Protein A2 (RPA2) Antibody [RPA2/4774]

Additional information

Catalog Number: 6118-MSM4
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
6118-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6118-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6118-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • IHC
  • WB

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Host
Mouse
Species Reactivity
Clone
RPA2/4774
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus, PML body
Gene Name
Positive Control
HeLa or HepG2 cells. Human tonsil (IHC)., MCF-7
Immunogen
Recombinant human full-length Replication Protein A2 (RPA2)protein
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
SwissProt
Chromosome Location
1p35.3
Mol. Weight of Antigen
32-34kDa
Alternate Names
Replication protein A 32 kDa subunit, Replication factor A protein 2, Replication protein A 34 kDa subunit, p32; p34; REPA2; Replication protein A 32kDa subunit; Replication protein A 34kDa subunit; Replication protein A2; RF-A protein 2; Rf-A2; RFA; RP-A p32; RP-A p34; RP21C; RPA32; RPA34; RpLP1; RpP2

Summary

Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

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