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Recombinant ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody [rZAP70/2046]

Additional information

Catalog Number: 7535-MSM8
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7535-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7535-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7535-MSM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody

Formalin-fixed, paraffin-embedded human lymphoma stained with ZAP70 Recombinant Mouse Monoclonal Antibody (rZAP70/2046).

Recombinant ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 Recombinant Mouse Monoclonal Antibody (rZAP70/2046).

Recombinant ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified ZAP70 Recombinant Mouse Monoclonal Antibody (rZAP70/2046). Confirmation of Integrity and Purity of Antibody.

ZAP70 Antibody in Western Blot (WB)

ZAP70 Antibody in Western Blot (WB)

Western Blot Analysis of Jurkat cell lysate using ZAP70 Recombinant Mouse Monoclonal Antibody (rZAP70/2046).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rZAP70/2046
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
ZAP70
Positive Control
Jurkat cells.Tonsil or lymph node.
Immunogen
Recombinant fragment (around aa247-382) of the human ZAP70 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Also contributes to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7535
SwissProt
P43403
Chromosome Location
2q11.2
Mol. Weight of Antigen
69.87kDa
Alternate Names
Tyrosine-protein kinase ZAP-70, ADMIO2|IMD48|SRK|STCD|STD|TZK|ZAP-70

Summary

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

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