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Recombinant TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer) Antibody [rNKX3.1/6620]

Additional information

Catalog Number: 4824-MSM19
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4824-MSM19-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4824-MSM19-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4824-MSM19-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer) Antibody

Formalin-fixed, paraffin-embedded human prostate stained with NKX3.1 Recombinant Mouse Monoclonal Antibody (rNKX3.1/6620).

Recombinant TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rNKX3.1/6620 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer) Antibody - Image 3

SDS-PAGE Analysis of Purified NKX3.1 Recombinant Mouse Monoclonal Antibody (rNKX3.1/6620). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rNKX3.1/6620
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
NKX3-1
Positive Control
Highly expressed in the prostate and at a lower level in the testis.
Immunogen
Recombinant fragment (around aa92-224) of the human NKX3.1 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Reactivity:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4824
SwissProt
Q99801
Chromosome Location
8p21.2
Mol. Weight of Antigen
26.35kDa
Alternate Names
Homeobox protein Nkx-3.1, BAPX2|NKX3|NKX3.1|NKX3A

Summary

NKX3.1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21.2, which frequently undergoes a loss of heterozygosity. NKX3.1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3.1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3.1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas.

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