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Recombinant Thrombomodulin / CD141 (Endothelial Cell Marker) Antibody [THBD/8188R]

In Stock
Catalog Number Formulation Size Price
7056-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7056-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7056-RBM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Thrombomodulin / CD141 (Endothelial Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human placenta stained with Thrombomodulin Recombinant Rabbit Monoclonal Antibody (THBD/8188R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Thrombomodulin / CD141 (Endothelial Cell Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified Thrombomodulin Recombinant Rabbit Monoclonal (THBD/8188R). Confirmation of Purity and Integrity of Antibody.

Thrombomodulin Antibody in Western Blot (WB)

Thrombomodulin Antibody in Western Blot (WB)

Western blot analysis of Human Lung tissue lysate using Thrombomodulin Rabbit Recombinant Monoclonal Antibody (THBD/8188R).

Thrombomodulin Antibody in Western Blot (WB)

Thrombomodulin Antibody in Western Blot (WB)

Western blot analysis of Lung tissue lysates of different species using Thrombomodulin Rabbit Recombinant Monoclonal Antibody (THBD/8188R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Membrane
Gene Name
THBD
Positive Control
Angiosarcoma|Bladder or Cervical Carcinoma.|Placenta, Lung
Immunogen
Recombinant fragment (around aa69-194) of the human CD141 protein (exact sequence is proprietary)
Alternate Names
Thrombomodulin, AHUS6|BDCA-3|BDCA3|CD141|THPH12|THRM|TM

Database Links

Entrez Gene ID
7056
SwissProt
P07204

Additional Information

Clone
THBD/8188R
Chromosome Location
20p11.21
Mol. Weight of Antigen
60.33kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All THBD Antibodies

Functions

  • Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed:10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed:29323190, PubMed:33836597, PubMed:9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed:26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed:15841214).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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