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Recombinant StAR (Steroidogenic Acute Regulator) (Leydig Cell Marker) Antibody [MSVA-740R]

In Stock
Catalog Number Formulation Size Price
6770-RBM14-P0
Purified Ab with BSA and Azide
20ug
$324.50
6770-RBM14-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
STAR Antibody in Numerous Tissues (IHC)

STAR Antibody in Numerous Tissues (IHC)

Steroidogenic acute regulatory protein, mitochondrial Rabbit Recombinant Monoclonal Antibody (MSVA-740R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone.It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Mitochondrion
Gene Name
STAR
Positive Control
Adrenal gland: A moderate to strong cytoplasmic StAR staining should be seen in the vast majority of adrenocortical cells.
Immunogen
Recombinant fragment (around aa 39-108) of human STAR protein (exact sequence is proprietary)
Alternate Names
Steroidogenic acute regulatory protein, mitochondrial, START domain-containing protein 1, Cholesterol trafficker; Luteinizing hormone induced protein; Mitochondrial steroid acute regulatory protein; StAR; StAR related lipid transfer (START) domain containing 1; StARD1; START domain-containing protein 1; Steroid acute regulatory protein; Steroidogenic acute regulator (STAR); Steroidogenic acute regulatory protein mitochondrial

Database Links

Entrez Gene ID
6770
SwissProt
P49675

Additional Information

Clone
MSVA-740R
Chromosome Location
8p11.23
Mol. Weight of Antigen
30kDa

Related Antibodies

View All STAR Antibodies

Functions

  • Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.

Key References

  • Lin, D., Sugawara, T., Strauss, J.F., 3rd, Clark, B.J., Stocco, D.M., Saenger, P., Rogol, A. and Miller, W.L. 1995. Role of steroidogenic acute regulatory protein in adrenal and gonadal steroidogenesis. Science 267: 1828-1831.
  • Pollack SE, et al. J Clin Endocrinol Metab. 1997; 82:4343-51.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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