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Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody [rSPTA1/1832]

Additional information

Catalog Number: 6708-MSM4
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
6708-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6708-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6708-MSM4-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 2

Western Blot Analysis of human K562 cell lysate using Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 3

Western Blot Analysis of human HeLa cell lysate using Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 4

Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

SPTA1 Antibody in SDS-PAGE

SPTA1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Spectrin alpha chain, erythrocytic 1 Recombinant Mouse Monoclonal Antibody (rSPTA1/1832). Confirmation of Purity and Integrity of Antibody.

SPTA1 Antibody in Western Blot (WB)

SPTA1 Antibody in Western Blot (WB)

Western Blot Analysis of human Kidney, Human Lung and K-562 lysates using Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rSPTA1/1832
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
SPTA1
Positive Control
Jurkat cells. Placenta or Tonsil., K562. HeLa, Kidney, Lung.
Immunogen
Recombinant fragment (around aa356-475) of the human Spectrin alpha, erythrocytic 1 (SPTA1) protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2617).
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Other Information

Functions
  • Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6708
SwissProt
P02549
Chromosome Location
1q23.1
Mol. Weight of Antigen
280.01kDa
Alternate Names
Spectrin alpha chain, erythrocytic 1, EL2|HPP|HS3|SPH3|SPTA

Summary

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

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