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Recombinant SPARC / Osteonectin Antibody [rOSTN/13279]

Additional information

Catalog Number: 6678-MSM20
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6678-MSM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6678-MSM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6678-MSM20-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Osteonectin Antibody in Immunohistochemistry (IHC (P))

Osteonectin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human testis stained with SPARC / Osteonectin Recombinant Mouse Monoclonal Antibody (r15G12). Inset: PBS instead of primary antibody; secondary only negative control.

Osteonectin Antibody in Western Blot (WB)

Osteonectin Antibody in Western Blot (WB)

Western blot analysis of Human Brain and U87 lysates using SPARC / Osteonectin Recombinant Mouse Monoclonal Antibody (r15G12).

Osteonectin Antibody in Western Blot (WB)

Osteonectin Antibody in Western Blot (WB)

Western blot analysis of A-375 cell lysate using SPARC / Osteonectin Recombinant Mouse Monoclonal Antibody (r15G12).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rOSTN/13279
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
SPARC
Positive Control
Human placenta, lymph node or spleen. JEG3, A-375 or A549 cell lysates.
Immunogen
Recombinant fragment corresponding to the c-terminal region of the human Osteonectin (O-Nectin) protein
Expression System
CHO
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6678
SwissProt
P09486
Chromosome Location
5q33.1
Mol. Weight of Antigen
34.63kDa
Alternate Names
SPARC, BM-40|OI17|ON|ONT

Summary

Osteonectin (ON), also known as BM-40 or SPARC (secreted protein, acidic and rich in cysteine) is a multifunctional glycoprotein (32.5 kD) involved with tissue mineralization as well as extracellular matrix modelling. It is the most abundant glycoprotein secreted by human osteoblasts in developing bone and odontoblasts of developing teeth. ON mRNA and protein have been detected in non-mineralized tissues such as steroid-producing cells of the adrenal glands, suprabasal layers of the epidermis, glomeruli in the kidney, bronchi of the lung, megakaryocytes and large vessels. This organ-specific distribution in non-mineralized tissues suggests a role during human development.

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