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Formalin-fixed, paraffin-embedded colon smooth muscle stained with SM-MHC Recombinant Mouse Monoclonal Antibody (rMYH11/8066). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified SM-MHC Recombinant Mouse Monoclonal Antibody (rMYH11/8066). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of Human Intestine and Human Stomach tissue lysates using SM-MHC Mouse Recombinant Monoclonal Antibody (rMYH11/8066).
Western Blot Analysis of Intestine and Stomach tissue lysates of different species using SM-MHC Mouse Recombinant Monoclonal Antibody (rMYH11/8066).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
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