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Recombinant Replication Protein A2 (RPA2) Antibody [RPA2/9851R]

In Stock
Catalog Number Formulation Size Price
6118-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6118-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6118-RBM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Replication Protein A2 Antibody in Western Blot (WB)

Replication Protein A2 Antibody in Western Blot (WB)

Western blot analysis of Human Brain, Human Heart, Human Kidney, Human Liver, Human Skeletal muscle, HeLa and K-562 lysates using Replication Protein A2 Rabbit Monoclonal Antibody (RPA2/9851R).

Replication Protein A2 Antibody in Western Blot (WB)

Replication Protein A2 Antibody in Western Blot (WB)

Western blot analysis of Brain, Heart, Kidney and Skeletal muscle tissue lysates of different species using Replication Protein A2 Rabbit Monoclonal Antibody (RPA2/9851R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus, PML body
Gene Name
RPA2
Positive Control
Human Brain, Human Heart, Human Kidney, Human Liver, Human Skeletal muscle, HeLa and K-562
Immunogen
Recombinant full-length human Replication Protein A2 (RPA2) protein
Alternate Names
Replication protein A 32 kDa subunit, REPA2|RP-A p32|RP-A p34|RPA32

Database Links

Entrez Gene ID
6118
SwissProt
P15927

Additional Information

Clone
RPA2/9851R
Chromosome Location
1p35.3
Mol. Weight of Antigen
29.25kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All RPA2 Antibodies

Functions

  • As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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