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Recombinant PRAME nuclear receptor transcriptional regulator Antibody [PRAME/8558R]

Additional information

Catalog Number: 23532-RBM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
23532-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
23532-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
23532-RBM6-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$559.00
23532-RBM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PRAME nuclear receptor transcriptional regulator Antibody

Formalin-fixed, paraffin-embedded human melanoma stained with PRAME Rabbit Monoclonal Antibody (PRAME/8558R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

Western blot analysis of SK-MEL-2 cell lysate using PRAME nuclear receptor transcriptional regulator Rabbit Recombinant Monoclonal Antibody (PRAME/8558R).

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

Western blot analysis of K-562 cell lysate using PRAME nuclear receptor transcriptional regulator Rabbit Recombinant Monoclonal Antibody (PRAME/8558R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
PRAME/8558R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Chromosome, Cytoplasm, Golgi apparatus, Nucleus
Gene Name
PRAME
Positive Control
SK_MEL-2, Human testis or melanoma.
Immunogen
Recombinant fragment (around aa100-509) of the human PRAME nuclear receptor transcriptional regulator protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed:21822215, PubMed:26138980). The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed:26138980). In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed:21822215). Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed:16179254).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
23532
SwissProt
P78395
Chromosome Location
22q11.22
Mol. Weight of Antigen
57.89kDa
Alternate Names
Melanoma antigen preferentially expressed in tumors, CT130|MAPE|OIP-4|OIP4

Summary

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

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