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Recombinant PRAME nuclear receptor transcriptional regulator Antibody [PRAME/8558R]

In Stock
Catalog Number Formulation Size Price
23532-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
23532-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
23532-RBM6-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$559.00
23532-RBM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PRAME nuclear receptor transcriptional regulator Antibody

Formalin-fixed, paraffin-embedded human melanoma stained with PRAME Rabbit Monoclonal Antibody (PRAME/8558R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

Western blot analysis of SK-MEL-2 cell lysate using PRAME nuclear receptor transcriptional regulator Rabbit Recombinant Monoclonal Antibody (PRAME/8558R).

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

Western blot analysis of K-562 cell lysate using PRAME nuclear receptor transcriptional regulator Rabbit Recombinant Monoclonal Antibody (PRAME/8558R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Chromosome, Cytoplasm, Golgi apparatus, Nucleus
Gene Name
PRAME
Positive Control
SK_MEL-2, Human testis or melanoma.
Immunogen
Recombinant fragment (around aa100-509) of the human PRAME nuclear receptor transcriptional regulator protein (exact sequence is proprietary)
Alternate Names
Melanoma antigen preferentially expressed in tumors, CT130|MAPE|OIP-4|OIP4

Database Links

Entrez Gene ID
23532
SwissProt
P78395

Additional Information

Clone
PRAME/8558R
Chromosome Location
22q11.22
Mol. Weight of Antigen
57.89kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All PRAME Antibodies

Functions

  • Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed:21822215, PubMed:26138980). The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed:26138980). In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed:21822215). Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed:16179254).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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