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Formalin-fixed, paraffin-embedded human lung stained with Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/7043R). Inset: PBS instead of primary antibody, secondary negative control.

SDS-PAGE Analysis of Purified Napsin A Mouse Monoclonal Antibody (NAPSA/7043R). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of Human Kidney lysate using Napsin A Rabbit Recombinant Monoclonal Antibody (NAPSA/7043R).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
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