Printable Datasheet

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody [CDH2/8815R]

In Stock
Catalog Number Formulation Size Price
1000-RBM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1000-RBM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1000-RBM12-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/8815R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody - Image 2

SDS-PAGE Analysis of Purified N-CAD Recombinant Rabbit Monoclonal Antibody (CDH2/8815R). Confirmation of Purity and Integrity of Antibody.

N-Cadherin Antibody in Western Blot (WB)

N-Cadherin Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain and Human Heart tissue lysates using N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/8815R).

N-Cadherin Antibody in Western Blot (WB)

N-Cadherin Antibody in Western Blot (WB)

Western Blot Analysis of Brain and Heart tissue lysates of different species using N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/8815R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Adherens junction, Cell junction, Cell membrane, Cell surface, Desmosome, Sarcolemma
Gene Name
CDH2
Immunogen
Recombinant fragment (around aa878-905) of the human N-Cadherin protein (exact sequence is proprietary)
Alternate Names
Cadherin-2, ACOGS|ADHD8|ARVD14|CD325|CDHN|CDw325|NCAD

Database Links

Entrez Gene ID
1000
SwissProt
P19022

Additional Information

Clone
CDH2/8815R
Chromosome Location
18q12.1
Mol. Weight of Antigen
99.81kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All CDH2 Antibodies

Functions

  • Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales