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Formalin-fixed, paraffin-embedded human skeletal muscle stained with Myoglobin Recombinant Mouse Monoclonal Antibody (rMB/2105).
SDS-PAGE Analysis. Purified Myoglobin Recombinant Mouse Monoclonal Antibody (rMB/2105). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Heart and Human Skeletal muscle tissue lysates using Myoglobin Recombinant Mouse Monoclonal Antibody (rMB/2105).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Myoglobin is a cytosolic oxygen-binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. Myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia.In combination with other striated muscle markers such as vimentin and myogenin, myoglobin is helpful in the identification of rhabdomyosarcoma and tumors with skeletal muscle differentiation. Reportedly, myoglobin is expressed on epithelial cancer cells due to changed metabolic and environmental conditions.
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