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Recombinant MYH7 Antibody [MYH7/9969R]

Additional information

Catalog Number: 4625-RBM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
4625-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4625-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4625-RBM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Myosin Heavy Chain 7 Antibody in Immunohistochemistry (IHC (P))

Myosin Heavy Chain 7 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Myosin Heavy Chain 7 (MYH7) Recombinant Rabbit Monoclonal Antibody (MYH7/9969R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant MYH7 Antibody - Image 2

SDS-PAGE Analysis of Purified MYH7 Mouse Monoclonal Antibody (MYH7/9184). Confirmation of Purity and Integrity of Antibody.

Myosin Heavy Chain 7 Antibody in Western Blot (WB)

Myosin Heavy Chain 7 Antibody in Western Blot (WB)

Western Blot Analysis of skeletal muscle tissue lysates of different species using Myosin Heavy Chain 7 Recombinant Rabbit Monoclonal Antibody (MYH7/9969R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
MYH7/9969R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Myofibril, Sarcomere
Gene Name
MYH7
Positive Control
Skeletal muscle.
Immunogen
Recombinant fragment corresponding to the n-terminal region of the human MYH7 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4625
SwissProt
P12883
Chromosome Location
14q11.2
Mol. Weight of Antigen
223.09kDa
Alternate Names
Myosin-7, CMD1S|CMH1|CMYO7A|CMYO7B|CMYP7A|CMYP7B|MPD1|MYHCB|SPMD|SPMM

Summary

Myosin heavy chains are ubiquitous Actin-based motor proteins that convert the chemical energy derived from ATP hydrolysis into the mechanical energy that drives diverse motile processes in eukaryotic cells, including cytokinesis, vesicular transport and cellular locomotion. Muscle myosin is a heterohexamer consisting of two myosin heavy chains and two associated nonidentical pairs of myosin light chains. The seven myosin heavy chain isoforms that predominate in mammalian skeletal muscles include two developmental isoforms, MHC-embryonic (MYH3) and MHC-perinatal (MYH8); three adult skeletal muscle isoforms, MHC IIa (MYH2), MHC IIb (MYH4) and MHC IIx/d (MYH1); and MHC-β/slow (MYH7 or MHC-β), which is also expressed in cardiac muscle. Research indicates that mutations of the MYH7 gene causes hypertrophic cardiomyopathy.

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