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Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).
Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).
Western Blot Analysis of human HeLa, A431, HepG2, HAP1, U937 cell lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).
SDS-PAGE Analysis of Purified MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of HT-29 and HEK-293 cell lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).
Western Blot Analysis of Human Brain, Human Heart, Human Kidney, Human Liver, Human Ovary and A431 lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Rabbit Brain, Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney, Rabbit Kidney, Human Ovary, Mouse Ovary, Rat Ovary, Hamster Ovary, Guinea pig Ovary, and Rabbit Ovary tissue lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).
Western Blot validation of anti-MTAP antibody. MTAP protein expression was compared in wild-type (WT) and MTAP shRNA Knockdown (KD) HeLa cells (30ug of total protein). Loading control is shown below.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a protein of 31kDa, which is identified as MTAP (5′-deoxy-5′-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
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