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Recombinant Interferon gamma (IFNG) Antibody [rIFNG/4467]

Additional information

Catalog Number: 3458-MSM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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Recombinant Interferon gamma (IFNG) Antibody

Formalin-fixed, paraffin-embedded human kidney stained with Interferon gamma Recombinant Mouse Monoclonal Antibody (rIFNG/4467).

Recombinant Interferon gamma (IFNG) Antibody - Image 2

SDS-PAGE Analysis of Purified IFNG Recombinant Mouse Monoclonal Antibody (rIFNG/4467). Confirmation of Purity and Integrity of Antibody.

Interferon gamma / IFNG Antibody in Western Blot (WB)

Interferon gamma / IFNG Antibody in Western Blot (WB)

Western blot analysis of A549 cell lysate using Interferon gamma Recombinant Mouse Monoclonal Antibody (rIFNG/4467).

  • IHC
  • WB
In Stock
Catalog Number Formulation Availability Size Price
3458-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
3458-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
3458-MSM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rIFNG/4467
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Secreted
Gene Name
IFNG
Positive Control
PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma. A549.
Immunogen
Recombinant full-length human Interferon gamma protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3458
SwissProt
P01579
Chromosome Location
12q15
Mol. Weight of Antigen
19.35kDa
Alternate Names
Interferon gamma, IFG|IFI|IMD69

Summary

Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN-纬, and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-纬. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-伪/尾 (type I interferon).

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