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Recombinant Interferon gamma (IFNG) Antibody [IFNG/13455R]

In Stock
Catalog Number Formulation Size Price
3458-RBM21-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3458-RBM21-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3458-RBM21-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Interferon gamma / IFNG Antibody in SDS-PAGE

Interferon gamma / IFNG Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Interferon gamma Recombinant Rabbit Monoclonal Antibody (IFNG/13455R). Confirmation of Purity and Integrity of Antibody.

Recombinant Interferon gamma (IFNG) Antibody - Image 2

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Interferon (IFN)- translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
IFNG
Positive Control
PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.
Immunogen
Recombinant fragment (around aa1-166) of the human Interferon gamma protein (exact sequence is proprietary)
Alternate Names
Interferon gamma, IFG|IFI|IMD69

Database Links

Entrez Gene ID
3458
SwissProt
P01579

Additional Information

Clone
IFNG/13455R
Chromosome Location
12q15
Mol. Weight of Antigen
19.35kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All IFNG Antibodies

Functions

  • Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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