Printable Datasheet

Recombinant Inhibin, alpha (INHA) (Gonadal Cell Marker) Antibody [INHA/8872R]

In Stock
Catalog Number Formulation Size Price
3623-RBM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3623-RBM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3623-RBM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
3623-RBM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3623-RBM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3623-RBM1-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Inhibin, alpha (INHA) (Gonadal Cell Marker) Antibody

IHC analysis of formalin-fixed, paraffin-embedded human testicular carcinoma. Stained with INHA/8872R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Inhibin, alpha (INHA) (Gonadal Cell Marker) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using INHA/8872R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

INHA Antibody in Western blot (WB)

INHA Antibody in Western blot (WB)

Western Blot Analysis of human Testis tissue lysate using INHA Rabbit Recombinant Monoclonal Antibody (INHA/8872R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a 47kDa protein, which is identified as alpha sub-unit of Inhibin. It is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH). Inhibin comprises two subunits, Inhibin A and Inhibin B. Each subunit consists of the same α subunit, covalently linked to 1 of 2 distinct subunits, β-α or β-β. Originally isolated from ovarian follicular fluid and characterized as a disulphide-linked dimeric glycoprotein, inhibin belongs to the transforming growth factor β (TFGβ) superfamily. Antibodies against Inhibin are useful in making a differentiation between adrenal cortical tumors and renal cell carcinoma. Sex cord stromal tumors of the ovary as well as trophoblastic tumors also demonstrate cytoplasmic positivity. Inhibin antibody is also used to make the differential diagnosis of intra-uterine vs. ectopic pregnancy in endometrial curetting.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
INHA
Positive Control
Human prostate or testis.
Immunogen
Recombinant fragment (around aa73-96) of the human Inhibin, alpha protein (exact sequence is proprietary)
Alternate Names
Inhibin alpha chain, -

Database Links

Entrez Gene ID
3623
SwissProt
P05111

Additional Information

Clone
INHA/8872R
Chromosome Location
2q35
Mol. Weight of Antigen
39.67kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All INHA Antibodies

Functions

  • Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins., Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor., Inhibin B is a dimer of alpha and beta-B that plays a crucial role in the regulation of the reproductive system by inhibiting the secretion of follicle-stimulating hormone (FSH) from the anterior pituitary gland. Thereby, maintains reproductive homeostasis in both males and females. Acts as a more potent suppressor of FSH release than inhibin A (By similarity). Functions as competitive receptor antagonist binding activin type II receptors with high affinity in the presence of the TGF-beta type III coreceptor/TGFBR3L (PubMed:34910520).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales