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Western Blot Analysis of MDA-MB-231 lysate using Histone H3 Recombinant Mouse Monoclonal Antibody (rHH3/9924)
Formalin-fixed, paraffin-embedded human tonsil stained with Histone H3 Recombinant Mouse Monoclonal Antibody (rHH3/9924). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human tonsil stained with Histone H3 Recombinant Mouse Monoclonal Antibody (rHH3/9924). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified Histone H3 Recombinant Mouse Monoclonal Antibody (rHH3/9924). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of HeLa, MDA-MB-231 and MCF7 cell lysates using Histone H3 Recombinant Mouse Monoclonal Antibody (rHH3/9924)
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark’s level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
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