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Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
Formalin-fixed, paraffin-embedded Rat Pancreas stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
Immunofluorescent staining of PFA-fixed HeLa cells using Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1) followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cellmembrane.
Western Blot Analysis of human heart tissue lysate using Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
Flow Cytometric Analysis of HeLa cells. Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
SDS-PAGE Analysis Purified Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human spleen tissue lysate using Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
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